mouse single-cell rna sequencing data  (Biotechnology Information)

Journal: Science (New York, N.Y.)

Article Title: In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes

doi: 10.1126/science.aaz6063

Figure Lengend Snippet: (A) Schematics of the in vivo Perturb-Seq platform, which introduces mutations in individual genes in utero at E12.5, followed by transcriptomic profiling of the cellular progeny of these perturbed cells at P7 via single-cell RNA sequencing (scRNA-seq). (B) tSNE of five major cell populations identified in the Perturb-Seq cells. (C) In vivo Perturb-Seq lentiviral vector carrying an mCherry reporter drives detectable expression within 24h, and can sparsely infect brain cells across many brain regions. Scale bar is 1000μm. (D) Cell-type analysis of in vivo Perturb-Seq of ASD/ND de novo risk genes. Canonical marker genes were used to identify major cell clusters (left), and cell-type distribution in each perturbation group (right). Negative control (GFP) is highlighted by a black rectangle. (E) tSNEs showing the subclusters of each of the five major cell types, identified by re-clustering each cell type separately.

Article Snippet: Based on mouse cortical single-cell RNA sequencing (scRNA-seq) data, the orthologs of these ASD/ND risk genes are expressed in diverse cell types ( fig. S2 ) (E18.5 data from the 10x Genomics public dataset ( 10 ); P7 data from this work).

Techniques: In Vivo, In Utero, RNA Sequencing, Plasmid Preparation, Expressing, Marker, Negative Control